By TRICLOSAN on Monday, July 1, 2002 - 05:04 pm:
Hi there,
I would appreciate any input on how to quantify TRICLOSAN in personal care products, mainly by HPLC.
Thank You!
TRICLOSAN
By Anonymous on Monday, July 1, 2002 - 05:47 pm:
Easy, my company does this everyday. I'll provide the basics without giving proprietary info. However, you'll need to validate yourself in your matrix if you use for QC or FDA stability. RP-18 column (type A silica OK), 65% acetonitrile, 35% water at acidic pH to keep phenolic protonated, UV detection at 280 nm. Flow about 1 ml/min for 4.6 mm i.d. columns. You could also use methanol in place of acetonitrile, use about 75% methanol.
By TRICLOSAN on Monday, July 8, 2002 - 11:52 am:
Thanks a lot for sharing!
TRICLOSAN
By vijay kumar verma on Sunday, June 6, 2004 - 11:54 pm:
Ihave just come across your site and it seems to be pretty helpful.I hope to use it more often Thanks for sharing your knowledge.
Vijay Kumar Verma
Senior Manager QA
P.I.Pharmaceuticals
By Hopefully helpfull on Saturday, June 26, 2004 - 07:04 am:
This is the second posting I have come across for Triclosan. Both empolyed a C-18 column. I have had great success with a C-8 column for many years with different detergent matrices. I have recently, due to lack of a C-8 column, back ordered, had to use a C-18. The only observation I have made is that the retention time and therefore analysis time for the C18 is double that for a C-8, 12 minutes versus 6 minutes. For a single inject of a single standard followed by a single injection for each of a couple of samples the difference is not significant. However, I have to make triplicate injections of a three point calibration standard set followed by dulicate injections of each sample. For me the C-8 has a great advantage.