By Bhaskar Reddy.R on Thursday, July 25, 2002 - 09:09 pm:
Dear All,
We are using copper sulfate buffer for the chiral separation of two enantiomers. Can any body advice me how to wash my HPLC system and analytical column after the analysis. Can I use diluted solution of EDTA for the washing of is it enough to wash with water and solvent as we are giving for reversed phase HPLC analysis.
By Anonymous on Friday, July 26, 2002 - 03:54 pm:
If it works, don't wash your column. Wash the HPLC with the usual stuff (methanol-water 50-50).
By Chris Pohl on Saturday, July 27, 2002 - 08:07 am:
The answer depends upon what format you are changing to and what sort of tubing you are using.
If you are switching to ion exchange, simple rinse steps are probably enough but if you are switching to reversed phase, I would recommend an EDTA rinse step, especially if you have any polymeric tubing upstream from the column since polymeric tubing has cation exchange sites on its surface which will retain traces of copper. Copper can also stick to silanols in your reversed phase column and significantly change the separation for any analytes with chelating potential. By the way, its best to adjust the EDTA pH to 6-7 to maiximze its complexing potential.