By Anonymous on Friday, August 2, 2002 - 01:37 am:
Hello,
I'd like to know what happens if I wash a diol column with pure water.
And more generally what are the differences between a silica column and a diol column.
Thanks a lot
Mark
By Anonymous on Monday, August 5, 2002 - 06:40 am:
A diol column works mainly by hydrogen bonding, just like silica does. Unlike silica, diol is not deactivated by small amounts of water. In fact, I've seen a few compounds that work well in reverse phase on diol.
By Anonymous on Thursday, August 8, 2002 - 08:40 am:
Mark,
Silica stationary phase is made only by silica.
Diol stationary phase is made by covalent bonding of diol groups on the support surface (generally silica).