By Anonymous on Friday, August 16, 2002 - 12:06 pm:
I am using a ZB-1 30 m x 0.530 mm x 5 µm column and I would like to know how to determine void volume for this column. Also, what is an acceptable capacity factor for this column.
By Anonymous on Friday, August 16, 2002 - 01:18 pm:
the volume of a cylinder is pi*radius*radius*length
do the math and you will determine the void volume of the column.
The sample capacity of this column is 100 to about 1000ng, depends upon the analyte.
(the void volume answer is 6.62cc but do the math anyway)
By Anonymous on Friday, August 16, 2002 - 10:12 pm:
Second method:
To determine void volume measure the flow rate through the column accurately.
Inject nitrogen for a tcd detector, methane for FID detector.
Determine time of elution.
Time * rate of flow = void volume
By Anonymous on Monday, August 19, 2002 - 02:03 pm:
Can anybody comment on the injection of Methane as an "unretained" peak to measure void volume and other parameters such as linear flow, etc?
By Anonymous on Tuesday, August 20, 2002 - 02:05 pm:
Assuming you are using capillary columns with non-plot or scot coatings, I cannot see any problems in using this technique correctly to give you the measured time of purging the column, as long as you are using it correctly.