Dear Moderator,

I'm finding difficulty in overcoming from tailing with one of our compound which has a Abs.max at 338nm.The compound is well eluting with 0.05M NDOP Buffer :ACN ::50:50 without any shift in RT in Hichrome RPB column. But there's a prominent tailing even at lesser pH with PO4 Buffer , TEA as an additive & 0.1% TFA :ACN Combinations.I would be highly thankful If I can be had any suggestion...

Thanks in Advance

Regards,

Syed Mustafa

Syed;

My first recommendation will be to try a new column of the same type. If this does not work, and the molecule you are working with has amine groups, then try some of the new columns recommended for amines, like the ACE 5C8 from MacMod.

Even with a highly deactivated column some compounds still tend to give tailing signals. In that case is when TEA is recommended to improve peak symmetry. I also suggest you keep the mobile phase simple at the beginning, perhaps 0.05M (NH4)2HPO4 at pH 2.5 and ACN.

Good Luck;

Benjamin

Syed,

what is the nature of your compound? Acid? Base? Neutral? Multiple ions?
Also, what is the pH?