Hello,

I am looking for some clinical uses of HPLC in special about preteins.
I know a hospital that perform assays in gel electroforesis for matabolical disorders such phenylketonuria and sickle cell disease .
can anyone point me a site,article,journal,book or methodology about clinical assays of proteins in body fluids for diagnostic?

thank you

Looks like nobody really in the know is answering this so here is a far flung shot at it (as an organic chemist in a med. school I try to stay clear of clinical chemistry, but have to keep a lame eye on it):
There are some companies that produce HPLC kits (Recipe, Munich; ChromSystems, Munich; Immundiagnostik, Bensheim; MedChrom, Heidelberg; BAS, West Lafayette; Alpha, Langen....). These are mostly for small molecules. Protein analyses are represented only sparsely, some specialty determinations like the one you mention, or hemoglobins, affinity chrom. of IgG subclasses, etc.
An explosion of protein chromatography, including HPLC, is currently taking place in connection with proteomics.
Much of clinical HPLC, especially if proteins are involved, appears to be estimation or even guess-work. Immunoassays are the mainstream, even though these yield numbers (always!) that may or may not represent the indicated substance.

Thank you very much.