By Anonymous on Wednesday, September 11, 2002 - 06:24 am:
Some water has come into a silica column because of using wrong mobile phase for a few mins.
What can I do now? Should I heat the column or just wash it by some solvents such as methaol?
Thanks!
By Anonymous on Wednesday, September 11, 2002 - 11:05 am:
If it was me, I'd flush through with isopropyl alcohol instead, the "usual" choice as an intermediate solvent between aqueous and non-polar solvents for HPLC.
By Uwe Neue on Wednesday, September 11, 2002 - 03:13 pm:
Not a problem! If there was no salt in the aqueous mobile phase, wash the column with an alcohol. IPA is probably best. If your mobile phase is a straight hydrocarbon, wash with an intermediate solvent such as methylene chloride next. If not, go to your mobile phase and reequilibrate.
By Anonymous on Thursday, September 12, 2002 - 07:08 am:
It seems ok now. Thank you both!
By Anonymous on Wednesday, February 26, 2003 - 06:52 am:
Water is irreversibility adsorbedonto silica. It can be removed by washing the column with 90/10/2.5 by volume of methylene chloride, acetic acid, and 2,2-dimethoxy-propane.