By pskar on Tuesday, September 17, 2002 - 03:21 am:
Can any body help me in qualifying the new HPLC columns when purchased newly.
By Anonymous on Tuesday, September 17, 2002 - 07:47 am:
I would compare results and performance to the columns you've been using, and document that.
By Anonymous on Tuesday, September 17, 2002 - 08:45 am:
Many columns are delivered with test mixture and test chromatogram, just inject this mixture with the recomended mobile phase, and compare with suppliers results.
By nic on Tuesday, September 17, 2002 - 06:51 pm:
We typically calculate N and compare to the suppliers reported N as per test method. N can be calculated by tangent, 5sigma or 2 sigma height. Go to Troubleshooting HPLC authored by the HPLC gods (snyder/Dolan), p91-92.
You will get some interlaboratory variation but overall we fall within +/-10% reported N.
I recall that Millenium has a add-on called system suitability which computes N.
By Anonymous on Tuesday, September 24, 2002 - 05:31 am:
you can go to nist web site and have a very good article on SRM870 STD FOR QUALIFYING A COLUMNS AND GIVEN A COMPARISION DATA OF 40 OR SO COLUMNS
OKEY
SHIRISH