Peak area units for linearity graph

Chromatography Forum: LC Archives: Peak area units for linearity graph
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Monday, September 23, 2002 - 07:08 pm:

We would like to know whether is correct to give units to a peak area or not.
We understand the peak area is an integration of the response obtained with respect to the time,in a limited interval of time. We are not sure if the peak area should be expressed as millivolts/Units of Absorbance, simply express it as a peak area with no units, or alternatively, as millivolts*second/Absorbance Units*second.

Our computer package is Millenium32.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Tuesday, September 24, 2002 - 05:36 am:

I believe the units for the area of a peak are usually expressed as microvolt-seconds.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By hinsbarlab on Tuesday, September 24, 2002 - 12:39 pm:

Most data analysis packages have provisions for converting the raw signal (mV) into a more meaningful unit (e.g., mAU). That way the absorbance value you see on the chromatogram matches what you see on the detector. Once converted, area would be expressed as (mAU x sec.) Gotta be careful to get the conversion factor right though. Check the specifications in your instrument manual.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By omarikhalid on Wednesday, April 28, 2004 - 06:03 am:

PEAK AREA = h x W½
the peak height (h) and peak width at half-height (W½)
so peak area = unit of signal which is mainly y-axis in the chromatogram X unit of time which is mainly x-axis in the chromatogram
peak area = milli or kilo volts (counts).minute or second (i.e: mV.S)


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