We analyse aminoglucosides with RP column, mobile phase with pentane sulfonic acid sodim salt, sodium sulfate, acetic acid and water, and detection with fluorescence detector.
But other tests prove enantiomeric compounds. How to choose a column to separate them?

Mila;

I think that you should examine the literature and try to find cases similar to yours. You mention that it is possible to detect your compounds by fluorescence, and therefore they must contain some large aromatic group.

Perhaps you can use ligand exhange chromatography (commonly employed for amino acids, and amino alcohols), or chiral crown-ether columns (employed for amino acids, peptides, and aromatic compounds with primary amine groups) for direct resolution. Or a derivatization approach with FLEC or other suitable reagent (it will form diastereomers with primary and secondary amine groups). With no more information, these are two approaches that look promising.

Good Luck;

Benjamin