Need a method to determine Triglyceride contents in Vegetable oil sample. USP method doesn't resolve peaks well.

Can anyone help? Many thanks.

My company has done intact triglycerides both by HPLC with ELSD detector (contact Alltech) and by high-temperature capillary GC. Most often stuff like this is saponified/esterified and fatty acid methyl esters assayed by GC.

we do this here with RPLC with a ACN/Isopropanol/Hexane mixture .total resolution of all peaks of olive and soya oil in 60 minutes

Are you using ECN technique?

Hi, Fabiano:

Thank you for your reply to my question. I am not familiar with ECN, what exactly is it?

In your method, do you have to connect two columns in a series as in the USOP method?

Thanks,

since is difficult have standard of all triglycerides combinations we use the ECN(Equivalent Carbon Nunber).this will sound like Kovats index for some one,but works very well .
we verify all peaks with LC-MS last year and it was ok.
what we do is run a satured chain triglyceride sequence and extrapolate the behavior of the insatured.
I made a mistake in the last post:we spent about 20 minutes in which run in a 5µ ODS 250x4,6 mm column for our tropical oil and 40 minutes for others with more satured chain .
the last uses of ECN that I read are about caracterization of olive oil geographic origin and triglyceride constitution in humna milk

the first is :
The potential of HPLC triglyceride profiles for the classification of Cretan olive oils, Food Chemistry, Volume 60, Issue 3, November 1997, Pages 425-432
Evangelia Stefanoudaki, Fani Kotsifaki and Aristidis Koutsaftakis

bye