Are there any limitations of using polypropylene vials in the GC/LC? Are they resistant to organic solvents such as hexane, ethanol, methanol and acetonitryle? I'm going to measure the contents of various phospho- and sphingolipids in the biological samples on GC (samples dissolved in hexane) and HPLC (samples dissolved in ethanol). Which vials are more suitable for my analyses: glass or polypropylene ones?

I've seen extractables come from PP vials when using ethanol, but the enhanced stability of the compound was worth it, compared to glass. Make sure you have adequate blanks that have spent at least as much time in the PP vials as your samples. I assume hexane would pose a larger problem for extractables. Also, the PP vials might not seal as well as glass, but that's a guess.

There several plastic resistance charts on the web such as:

http://nalgenelab.nalgenunc.com/scripts/nalge/nalgene/select/chem/index.asp

all commercial PPs contains phenol and phosphite additives such as: irganox 1010 and irgafos 168

Thanks a lot for the answers.