By Anonymous on Friday, October 11, 2002 - 09:24 am:
Does anyone know where I can find an HPLC method to separe the 6 isomers of Dichlorobenzoic Acid ?
Thanks
By maris on Saturday, October 12, 2002 - 12:45 am:
Try "Cosmosil PYE" or "Cosmosil NPE" columns.We received very good separation for "positional" isomers.
By Benjamin on Monday, October 14, 2002 - 11:11 am:
Dear Anonymus;
In my experience, the best columns for this type of separations are the Fluophases PFP and RP from Keystone. I am not sure you will get baseline resolution of all isomers, but it will be close.
Good Luck;
Benjamin
By maris on Monday, October 14, 2002 - 11:27 pm:
Dear Benjamin & Anonimus,
In our experiense the separation power of "Cosmosil PYE/NPE" is over the Flourophases, we get exellent resolution for series of Di/Tri-Cl aromatics, much better than with "Flouro".
By Anonymous on Friday, October 18, 2002 - 05:02 am:
For the separation of "positional" isomers there are some applications at www.synaptec-gmbh.de
By maris on Friday, October 18, 2002 - 05:36 am:
Somebody deleted a sufficient part of our disscution (starting from 14.10 ), why?????
By Anonymous on Friday, October 18, 2002 - 08:01 am:
I don't know Maris.
But I have to tell you that I have resolved the problem with a C-18 (maybe we can't say brands !!!) 25 x 4.6 at 220 nm in a gradient conditions with ACN and phosphate buffer at pH 2.0, oven at 45 ºC.
Thanks all for your comments
Anonymous Original
By maris on Friday, October 18, 2002 - 12:09 pm: