By Anonymous on Tuesday, October 22, 2002 - 11:56 pm:
Deal all,
we have a new product that comprised of Niacin and Lovastatin . I want to analyse this two compounds at the same chromatogram and I hope it won't take a long time. but I found it is a little difficult . because Niacin is water soluable very easily , however Lovastatin is unsoluable .so the Niacin is weak reserved and lovastatin is relatively strong reserved. I have tried ion-pair chromatography . but they are not so ideal . do anybody have a good sugestion for this?
Thanks !
Gavin Chan
By Mel on Wednesday, October 23, 2002 - 11:41 am:
I found this reference:
Determination of lovastatin concentration by reverse-phase liquid chromatography
Qingdao Huagong Xueyuan Xuebao 20 (1999) 353-356 Li, L. and Wu, J. C.A., C.A
Technique: LC;
I don't know if it will help or not but it may be worth a look.
Melissa
By Mel on Wednesday, October 23, 2002 - 11:54 am:
Have you looked at the USP method for lovastatin? It uses a C18 column with ACN and buffer. From the USP methods of both nicacin and lovastatin it looks like you could assay them together.
Just my opinion,
Melissa
By Anonymous on Friday, February 21, 2003 - 12:27 am:
Dear Melissa ,
I have not been on the Chromforum for a long time, so I didn't see your reply.Thank you for your warm heart .
Actually , I have tried to use the methods for Niacin and Lovastatin in the USP.but they didn't work. Niacin just reserve weakly.evenly with CN column and Ion-pair and pH far away from the pKa.
I am continuing to develope this method now, so anyone's advice is welcomed.
Gavin