By Memerick on Friday, October 25, 2002 - 10:55 am:
I have upgraded the millennium software to Empower and have planed to use it for all of our calculations. But, I have not had very good support from Waters in figuring out how to program the run to calculate my assay values. Is there any one out there that knows how to program waters software to calculate the assay of the samples based off of bracketing standards? Any help would be appreciated.
By Anonymous on Friday, October 25, 2002 - 11:09 am:
Did you check the on-line help? I just checked and there was some fairly detailed how to information there.
By Memerick on Friday, October 25, 2002 - 12:18 pm:
I went there and they had details all the way up to how to enter the data so the results would be calculated. The website says it can be done. I think they want people to pay for there class.
By Anonymous on Friday, October 25, 2002 - 12:42 pm:
What do you mean when you say "how to enter the data"?
By Tom Mizukami on Friday, October 25, 2002 - 12:47 pm:
Hi Memerick,
We haven't upgraded to Empower yet, but we calculate assay based on bracketing standards using Millennium 4.0 using a custom calculation that I can detail. Let me know if your question is specific to Empower or general to Millennium.
By Memerick on Monday, October 28, 2002 - 01:37 pm:
The calculations should be the same on Empower as it is in Millennium. We have used Millennium for a while but had never used it to do calculations. Going from the point of just injecting the sample and getting the area count too calculation off the bracketing standards are where we need help. I have tried to put some weights in the slots for the sample and standards but it never seems to calculate right. If I could have an example of a run from start to finish I know I could do it. I do not know what info you can give me. But any help would be great.
By Tom Mizukami on Monday, October 28, 2002 - 03:28 pm:
Hi Memerick,
We just use the label field in the sample set. Lets say you have five brackets, we would label the standards used to quantify std1-std6. The samples in bracket 1 would be labeled unk1, and so on for all of the brackets. After the last standard for the first bracket we insert three lines, clear calibration, calibrate (std1, std2), quantitate (unk1), repeat for all brackets.
I hope that is clear enough to follow, if you are still having problems e-mail me and I can send you some screen shots.
By Andreas Neumaier on Wednesday, November 27, 2002 - 01:26 am:
If you want to calculate results with the Waters Empower/Millennium software, you'll have to fill in the following colums in
QuickSet:
- Sample weight
- Dilution
- Function (inject standards, inject samples, clear calibration, calibrate, quantitate)
- Label (s1, s2, s3, s4,... for calibration solution / p1, p2, p3, p4, ... for samples)
- Amounts (the purity of the standards, no entrance for samples necessary)
Processing method (compounent table):
- Average by (Level for two or more points of calibration, none for one point)
- Sample value type (amount)
for the peak(s) you want to quantitate:
- peak name
- x value (amount)
- y value (area - or if you run a gradient: height)
- fit (linear through zero)
- weighting (none)
- default pk (yes)
Example for a sample set method (some colums I left out):
(sample type -- label -- processing)
inject standard -- s1 -- don't process or report
inject sample -- p1 -- don't process or report
...
inject sample -- p1 -- don't process or report
inject standard -- s2 -- don't process or report
(sample type -- label reference -- processing)
clear calibration -- no entry -- normal
calibrate -- s1 s2 -- don't report
quantitate -- p1 -- don't report
report -- p1 -- normal
make sure you use always the SAME processing method for clear calibration/calibrate/quantitate.
Don't forget to fill in your amounts in QuickSet.
BTW: I'm working with Millennium 3.05.01. There should be no changes for newer versions...