By Anonymous on Thursday, November 7, 2002 - 08:09 am:
I'm looking for a way to separate 'glucose' and and 'triacetyl-d-glucal' from '2-deoxy-d-glucose'. Does anyone have an idea or two for how to get this started?
By M on Thursday, November 7, 2002 - 11:30 am:
I found this reference, hope it helps.
9. Simultaneous determination of 2-deoxy-D-glucose and D-glucose in rat serum by high-performance liquid chromatography with post-column fluorescence derivatization.
Chem. Pharm. Bull. 38 (1990) 963-965 Umegae, Y., Nohta, H. and Ohkura, Y.
Melissa
By Uwe Neue on Thursday, November 7, 2002 - 04:07 pm:
What detectors do you have? The common way to try a separation of sugars and related compounds is through HILIC on an amino column, but a silica column might do the job too.
By dan on Friday, November 8, 2002 - 08:03 am:
Uwe, I have an Organic Acids column, and a Carbohydrate column, as well as C8's, C18's, and CN type. For detectors, I have variable UV and an RI. How is HILIC different than HPLC?
Dan
By d on Friday, November 8, 2002 - 08:08 am:
Melissa - I appreciate the reference. It confirms another bit of info I had previous. If you run across anything else, I could sure use it. Thanks.
Dan
By Uwe Neue on Friday, November 8, 2002 - 10:30 am:
HILIC is hydrophilic interaction chromatography, and is the way you use a carbohydrate column: acetonitrile water, with decreasing retention as you increase the water content.
The carbohydrate column should do fine, assuming that it is the amino type that uses acetonitrile-water for the separation. Have you tried this one yet? If you have not yet started, try 75% acetonitrile and 25% water. If you got too much retention, increase the water content and vice versa. If it did not work for you, let us know what the results were, and maybe we can see how we can improve things.
By dan on Friday, November 8, 2002 - 11:33 am:
Uwe,
I actually haven't tried anything yet . . . just getting some ideas. This project is still in the quotation phase. I was planning on trying the carbohydrate column setup first, but I'm not real confident even it will separate these compounds. It will be interesting to see what happens. Thanks so much for your help. I will let you know what happens.
Dan
By Einar Pontén - SeQuant AB on Tuesday, November 12, 2002 - 02:52 pm:
Dan, Since you still are considering options the ZIC™-HILIC column could be an alternative. This phase bears covalently bound and permanent zwitterionic functional groups, which means that it is highly hydrophilic, but still neutral in the entire pH range.
By d on Thursday, November 14, 2002 - 07:24 am:
Einar - I haven't heard of that brand column; I'll keep it in mind if my columns aren't working. Thanks for the suggestion.
Dan