By adam goldstein on Thursday, November 21, 2002 - 12:49 pm:
currently we are measuring height divided by with for a large scale DEAE column 63 cm, but I do not like this measurment, I feel that b/a is a much better indicator of bed degredation etc.
What do others feel? any journals or paper you know of that I can use a fuel for QA?
adam goldstein, wyeth 919 775 7100 x4113
By Anonymous on Thursday, November 21, 2002 - 02:50 pm:
Height divided by width will depend on retention also. Is this a gradient?
By adam goldstein on Monday, November 25, 2002 - 06:25 am:
No this is not a gradient , it is a step elution on a 63cm DEAE, currently we measure h / width, and the chart speed is too slow, and the measurements are impossible and I'm not sure they are meaningful even if we speed up the chart.
Isn't b/a a better indicator?
By Anonymous on Monday, November 25, 2002 - 03:40 pm:
b/a give you info on tailing, but not if the peak has become wider.
I think a simple width measurement would do best.