By clenh2o on Sunday, November 24, 2002 - 08:57 am:
I am a student who has a project to separate the cis- and trans- isomers of tamoxifen. I need help with column and mobile phase selection. My goal is simply to determine the ratio of formation of isomers during stereospecific vs non-stereospecific syntheses. Method development is killing me lab-time wise and I really need some help at this point.
Keep in mind that the HPLC I am using does not have a column sleeve to adjust the temperature. Everything I have done so far has been at room temp. If this is my big error, I CAN reconfigure my system.
If anyone has any suggestions on this topic I would greatly appreciate it.
By Melissa on Monday, November 25, 2002 - 07:34 am:
I did a search and found these references. All are HPLC, some with flourescence or MS detection. At least you can use the separation conditions.
Husain, S., Alvi, S.N. and Nageswara Rao, R.
Separation and determination of E- and Z-isomers of tamoxifen by ion-pair high-performance liquid chromatography
Anal. Lett., 27 (1994) 2485-2497
Fried, K.M. and Wainer, I.W.
Direct determination of tamoxifen and its four major metabolites in plasma using coupled column high-performance liquid chromatography
J. Chromatogr. B, 655 (1994) 261-268
Lim, C.K., Chow, L.C.L., Yuan, Z.-X. and Smith, L.L.
High performance liquid chromatography of tamoxifen and metabolites in plasma and tissues
Biomed. Chromatogr., 7 (1993) 311-314
Li, X.-F., Carter, S., Dovichi, N.J., Zhao, J.Y., Kovarik, P. and Sakuma, T.
Analysis of tamoxifen and its metabolites in synthetic gastric fluid digests and urine samples using high-performance liquid chromatography with electrospray mass spectrometry
J. Chromatogr. A, 914 (2001) 5-12
El-Yazigi, A. and Legayada, E.
Direct liquid chromatographic micro-measurement of tamoxifen in plasma of cancer patients
J. Chromatogr. B, 691 (1997) 457-462
Merle, O., Faure, N., Guitton, J., Burke, M.D. and Ollagnier, M.
Solid phase extraction and high performance liquid chromatography of tamoxifen and its demethylated metabolites in plasma
Anal. Lett., 31 (1998) 2679-2690
Merle, O., Guitton, J., Burke, M.D. and Ollagnier, M.
Rapid and simple method to determine tamoxifen and its major metabolites in human liver microsomes by high performance liquid chromatography with fluorescence detection
Anal. Lett., 31 (1998) 2067-2076
Koskinen, M., Rajaniemi, H. and Hemminki, K.
Analysis of tamoxifen-induced DNA adducts by 32P-postlabelling assay using different chromatographic techniques
J. Chromatogr. B, 691 (1997) 155-160
MacCallum, J., Cummings, J., Dixon, J.M. and Miller, W.R.
Solid-phase extraction and high-performance liquid chromatographic determination of tamoxifen and its major metabolites in breast tumour tissues
J. Chromatogr. B, 698 (1997) 269-275
MacCallum, J., Cummings, J., Dixon, J.M. and Miller, W.R.
Solid-phase extraction and high-performance liquid chromatographic determination of tamoxifen and its major metabolites in plasma
J. Chromatogr. B, 678 (1996) 317-323
Jones, R.M., Yuan, Z.-X., Lamb, J.H. and Lim, C.K.
On-line high-performance liquid chromatographic-electrospray ionization mass spectrometric method for the study of tamoxifen metabolism
J. Chromatogr. A, 722 (1996) 249-255
If you want to know where I found these references, contact me.
Melissa
By Anonymous on Monday, November 25, 2002 - 10:40 am:
Try this:
http://www2.waters.com/watprod.nsf/wwwWebList/?SearchView&Query=tamoxifen