By Anonymous on Wednesday, December 4, 2002 - 04:41 am:
Hallo, greetings from the Old Continent.
I am about to start working with the method for vitamin A according to the European Pharmacopoeia (EP 4.2 Vitamin A concentrate (oily form), synthetic; page 2814; assay-method B). The reference solution is made with retinol acetate CRS. My sample, however, is retinol palmitate. My question is: which substance am I suppose to use?
By the way, could you point me to a good supplier of this standards (in Europe)?
Also concerning the above analysis: do I have to be worried about tocopherol being present in my sample (EP uses butylhydroxytoluen as a antioxidant), will it interfere with my separation?
Thank you!
By Alex buske on Thursday, December 5, 2002 - 03:08 am:
As written in the text retinol palmitate and retinol acetat are saponified during workup.
The whole method works only to measure total retinol.
Regarding tocopherol I dont know. Does it basorb at 320nm?