By Anonymous on Tuesday, December 10, 2002 - 06:38 am:
Hi to all,
does someone know about an HPLC analytical method [using UV-VIS detector]of DONEPEZIL(ARICEPT)?
Thanks in advance
By Jan on Friday, January 3, 2003 - 03:24 am:
Hello Anonymous,
Pfizer uses Inertsil ODS-2 5 µm 150 x 4.6 at 35°C. The mobile phase is prepared as follows: 2.5 g sodium decanesulfonate in 650 ml water. Add 1 ml of 70% perchloric acid and 350 ml acetonitrile. Adjust apparent pH to 2.5. Flow 1.4 ml/min. 271 nm. Inject 20 µl of 0.2 mg/ml donezepil HCl in dilution solvent (0.1 N HCl - Methanol 1:3). Donezepil elutes after appr. 12 min.
Hope it works
Jan
By Anonymous on Friday, January 3, 2003 - 05:39 am:
u search in chemweb.com ,merhods available.all the best.
By Anonymous on Friday, January 3, 2003 - 05:42 am:
Adjust pH after adding acetonitrile? I thought, that we should measure pH in aqueous phase before mixing with organic solvent.
Could you explain this, for I’m new in HPLC, and getting confused.
Have a great 2003!!!
By Jan on Friday, January 3, 2003 - 06:24 am:
I admit adjusting pH before adding organic sovent is more staightforward. Anyway, I believe this particular method was developed by adjusting pH AFTER adding acetonitrile (that's why I wrote apparent pH). John Dolan had a very interesting column on this in the November or December 2002 issue of LC GC (a monthly published FREE journal, and available online)!
http://www.chromatographyonline.com/lcgc/data/articlestandard/lcgc/462002/38298/article.pdf
By Anonymous on Friday, January 3, 2003 - 06:42 am:
Jan:
I work in the industry. Method details are company property. Giving confidential info to third party could cause trouble. I appreciate your kindness but you might be more careful in the future.
Best regards.
By Anonymous on Tuesday, March 9, 2004 - 06:14 am:
hplc method for purity determination of moxifloxacin and gatifloxacin along with their acid and esters ( ie., with out their side chains re., nonane and 1 methyl piperizine)
By aniket a naik on Monday, June 21, 2004 - 12:31 am:
does anyone know TLC method for analysis of donepezil.
Thank you
aniket