By Anonymous on Monday, December 16, 2002 - 08:55 am:
Hi,
I need a Capillary HPLC system for LC/MS/MS.
Anyone who has experience in this area pls give some advices on the selection of such a system?
Stability and reproducibility are most important for me, also the accuracy of flow rate is very important.
Thanks a lot.
By Anonymous on Wednesday, December 18, 2002 - 01:56 pm:
You can check out the Rheos 2000 with the CPS low flow module from LEAP Technologies. www.leaptec.com.
By Luke on Friday, January 17, 2003 - 12:05 pm:
Hello Anonymous,
You can look at the Gilson 350 micro pumps. The system really depends on your application. Are you just looking for pumps? Are you going to be doing straight injections onto MS or will there be column switching applications involved. If so, what method of columns switching would you be doing? What flow rates are you interested in running at? Do you want to use a flow splitter or are you interested in True micro and nano LC (True nanoliter flow rates generated without splitting)?
Why is accuracy of flow rate important to you? Does the flow rate accuracy really matter? In my oppinion, the actual flow rate generated is irrelevant as long as it provide a consistent flow rate that gives adequate pressure for my 75 or 100 um column and that the gradient compositions are accurate. whether it's pumping 200 or 250 nl per minute doesn't seem that critical as long as it's consistent.
Let me know if you would like to discuss further.