By Anonymous on Saturday, January 4, 2003 - 07:37 am:
I'm trying to desalt peptide mixture by using C18 column. I usually use acetonitrile-water-TFA solutions as buffers. I want to know what's the correct procedures for desalting. thanks a lot.
By Anonymous on Sunday, January 5, 2003 - 07:34 pm:
This is done better on a C18 SPE, or even better on a non-silica SPE like Oasis HLB from Waters. Load the sample, wash with water, then elute with an organic solvent. Finally, evaporate the organic solvent.
By H W Mueller on Thursday, January 9, 2003 - 01:13 am:
Generally, the most simple method to manipulate salt content is via ultrafiltration or dialysis.