By Markus Schuett on Tuesday, January 7, 2003 - 06:56 am:
Hi all...
...i have a question...does everybody have any experience in seperating fully protected peptides containing 8 aminoacids like His(Trt)/Lys(Boc)/Cys(Trt) from ide products of syntheses on an hplc machine...till now i tried it with an macherey&nagel reverse phase c 18 preparative column, but nearly nothing came out...my eluants were h20 and acetonitril...i think i am just allowed to use this two buffers...has anybody an idea, how i can seperate something?...sorry of the english...i am from germany...thanks for your help!
CU
Markus
By Anonymous on Tuesday, January 7, 2003 - 04:09 pm:
use the same packing material in an analytical column first and work out the conditions.
You may need to add an acid, such as trifluoroacetic acid.
By Anonymous on Wednesday, January 8, 2003 - 08:48 am:
When you say nothing came out, you mean you didn't find any peak, or everything was eluting in the front of you chromatogram?