By Anonymous on Thursday, January 9, 2003 - 10:41 pm:
any one has a idea about hplc method of anlaysis for metformin +gliclazide in combination
as metformin is water soluble and gliclazide is insoulble metformin is eluting almost in dead volume in almost all type of reverse phase columns without ionpair.
is that ion pair is las solution for this no one has column which will elute metformin at 5 6 min and gliclazide at 9 10 min .
By Anonymous on Friday, January 10, 2003 - 04:17 pm:
How about ion exchange or HILIC on a silica column? (I do not know what gliglazide is, but it sounds as if it has some nitrogen on it)
By Anonymous on Thursday, February 6, 2003 - 04:01 am:
Try this article J. Pharm. Biomed. Anal. 2001 Apr;25(1):77-84.
By Anonymous on Thursday, January 22, 2004 - 03:02 am:
HPLC method for Metformin Analysis
By Erisa Owino on Monday, May 3, 2004 - 08:42 am:
I would appreciate information on a suitable HPLC method for metformin. We have student projects to do comparative studies of different brands
By Uwe Neue on Thursday, May 6, 2004 - 08:13 pm:
For such a polar compound, I propose a HILIC method on a HILIC silica column. I suggest UV detection in the low UV, with a gradient from 90% acetonitrile to 50% acetonitrile with pH control. The pH control is the item to play with to manipulate retention. I would start with phosphate at pH 2 and pH 7.