By Andrea Applegreen on Thursday, May 13, 1999 - 01:01 pm:
Is it a good idea to wash a column with 100% water after running samples with a mobile phase of acetonitrile:0.02%TFA. The gradient starts with 10% acetonitrile to 45%.
By Russell Rondeau on Thursday, May 13, 1999 - 01:58 pm:
Andrea, it is my understanding that washing a column with 100% water will collapse the column packing. You should keep some organic in there, 5-10% min. Then wash w/ a strong organic to restore or stand the packing back-up.
By JD on Friday, May 14, 1999 - 02:31 pm:
See a good discussion of this in April 99 LC Troubleshooting in LC/GC.
By srp1002001@yahoo.co.in on Sunday, April 11, 2004 - 09:04 am:
After completion of analysis it is not wrong to
wash the column with the 100% aqueous phase.
then restore the column in organic solvent.