Please help me

Chromatography Forum: LC Archives: Please help me
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Monday, January 20, 2003 - 04:39 am:

I'm working with purification of some extra
cellular metabolites from yeast. The substance
binds strongly with DEAE-cellulofine but i failed
to elute the substance while using NaCl (0~1 M).
The substance is pH stable from 3~9. I have used
buffer with pH 7~8.8 and always it happened to
bind stronly but does not elute.


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