This is a platinum complex with lactone ring. For HPLC analysis it will be dissolved in water. As expected it is hydrolysing to give two impurities. The hydrolysis is progressing linearly posing lot of problems. Samples need to be analysed as soon as they are made up which may not be practical. To improve the solution stability several buffers in the pH range of 3.0 to 8.0 were tried but of no use. The main problem here is one of the hydrolysis impurities is reacting with the anion present in solution and forming some other complex. Even mixtures of organic solvents(like methanol, ethanol, isopropanol and acetonitrile) in water were tried but same problem mentioned above was observed. The compound is soluble in water but sparingly in organic solvents. All the experiments were tried at lower temp of 4C.

The problem seems to be something to do with the reactivity of Platinum compounds.

Any ideas to improve the stabilty of this compound welcome.

Thanks in ADVANCE.

How about an assay in a non-aqueous medium? You do not need a lot of solubility.

I forgot to mention that I have no problem with the assay. I have problems with impurities content only

Thanks in Advance