How to separate NaCl from desired substance after ion exchange column?

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Top of pagePrevious messageNext messageBottom of pageLink to this message  By Rabbi on Tuesday, January 21, 2003 - 02:30 am:

Hello,
I'd been working with purification of some
metabolite that were produced extra cellularly.
To purify the substance, i used ion exchange
chromatogram and gel filtration. Now the problem
i face that the substance is along with NaCl and
i can't isolate this NaCl from my desired
substance. The probable molecular mass of the
substance is very low. Would you please suggest
me, how can i isolate my substance?


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Tuesday, January 21, 2003 - 03:14 am:

Try in a different mode.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Tuesday, January 21, 2003 - 08:33 am:

I've successfully used Alltech silver Maxi-Clean cartridges to remove chlorides prior to ion chromatography; these attach to the end of a syringe just like a filter unit.


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Rabbi on Tuesday, January 21, 2003 - 06:12 pm:

Thanks for your kind suggestion.
Would you pls. provide me Ordering information
for those cartridges.
Thanks again


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous 2 on Wednesday, January 22, 2003 - 08:06 am:

Alltech USA 1-800-255-8324 or www.allltechweb.com
Alltech calls these IC Resin SPE columns and cartridges. Vacuum manifold IC-Ag columns (0.5 ml) #105050 or #140258 (1.5 ml). Syringe luer Maxi-clean IC-Ag cartridges (0.5 ml) #30266 or #30258 (1.5 ml).


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Rabbi on Wednesday, January 22, 2003 - 05:32 pm:

Hi anonymous
Thanks for your kind notice.
Hope this will find my exp.a success.
Thanks again.


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