By Bobby on Tuesday, January 21, 2003 - 06:25 pm:
I'm working with purification of some extra
cellular biological substance produced by yeast.
The cell free cultured supernatant binds strongly
with DEAE-cellulofine but i failed to elute the
substance while using NaCl (0~2 M) in a test tube
method. The activity of the substance is pH
stable from 3~9. I have used different buffer pH
5.5~8.8 (20mM). What should i try next to desorb
the substance?