I've been asked to develop a method to determine the levels of CPC - cetylpyridinium chloride, in toothpaste and mouthwash formulations by HPLC. I am a little pressed for time, and more of a GCMS guy than HPLC.

I imagine I will use a C18 column, MeOH/PO4 aqueous buffer mobile phase, and UV detection. I think I should be able to find the CPC peak no problem, but will I need to do some type of SPE cleanup to separate the CPC from sorbitol, saccharin, glycerine etc.

It seems to me there has got to be some work done in this area already.

Can anybody help me get started?

Thanks,

Mike

mouthwash can be injected directly. No problem. With the toothpaste, you got to get rid of the solids. The rest of the stuff can be injected directly. glycerin, sorbitol etc are nothing to worry about. They break through early, and are invisible in the UV used to detect CPC

Try this link, if it doesn't work, it is to the Waters Application Library. Do a search on cetylpyridinium chloride and Voila!

http://www2.waters.com/watprod.nsf/wwwWebList/?SearchView&Query=cetylpyridinium%20chloride