By Anonymous on Thursday, January 23, 2003 - 03:57 am:
Does someone know about an HPLC method of ITRACONAZOLE using UV detector? (the method I'm using is not working!)
By Anonymous on Friday, January 24, 2003 - 08:16 am:
Can you give more details of your sample and what method at present you using, if it is possible ?
By Anonymous on Monday, February 3, 2003 - 03:48 am:
Hi anon.
I'm using RP-18 column, mobile phase: MeCN: water: DEA 60:40:0.05, detector: 263nm.
The samples contain Itraconazole and some kind of a polymer in water.
By Anonymous on Monday, February 3, 2003 - 10:09 am:
A quick search of the Waters site and there are 6 references. You should at least try a search first. These are all from serum, but they may give you a place to start.
By Anonymous on Monday, February 3, 2003 - 03:52 pm:
If you haven't killed your column yet with the DEA...
By Anonymous on Tuesday, February 4, 2003 - 12:51 am:
Thank u Anon(and my column is alive for now).
By Anonymous on Tuesday, February 4, 2003 - 04:01 am:
you mean that DEA... is going to wash the stationary phase, DEA... is going to help in getting better peak shape, sometimes noisy base line and decreaes the RT also
do you have another information ?
By Anonymous on Tuesday, February 4, 2003 - 11:19 pm:
The mentioned method is according to a method for formulations described in the book "HPLC methods for pharmaceutical analysis".
Don't know why they use DEA (starting to belive that we can do without it).
By Anonymous on Wednesday, February 5, 2003 - 11:26 am:
What's the pH?
By Anonymous on Wednesday, February 5, 2003 - 11:06 pm:
Much too high, >11.
By Anonymous on Thursday, February 6, 2003 - 04:06 am:
Don;t go for PH 11, THAT;S TOO MUCH, SOME TIMES YOUR STATIONARY PHASE IS GOING TO WASH OUT, I WOULD GO FOR < 7.0, YOU NEET WORK ON IT,
GENERAL USE IS TO GET THE BETTWER PEAK SHAPE( DEA) TO BASIFY ONLY, I DONT THINK SO NOTHING CHEMISTRY INVOLED IN IT. BETTTER TO KNOW DETIALS
GOOD LUCK
By Anonymous on Monday, February 10, 2003 - 07:00 am: