By Merja Lyytikainen on Thursday, January 23, 2003 - 06:50 am:
Can somebody help me...?!
We are setting up a method for PAHs with HPLC. The column is C-18 (25 cm, 4.6 mm) and we are using acetonitrile and water as mobile phase (2 ml/min). We have purchased a standard mixture of PAHs (from Dr. Ehrenstorfer, PAH 18 mix), which includes EPA 610 PAHs plus perylene.
The problem is that we can't figure out the retention order of the compounds (certificate has only GC-chromatogram in it). I have found examples of chromatograms for 16 EPA 610 compounds, but none with perylene included. The method is needed ASAP and we don't have pure single compounds in the storage. Ordering them would take weeks in the worst case. The manufacturer has not replied to my enquiry about the retention times.
Therefore, I was hoping that someone would know the retention time of perylene in comparison to other PAHs (e.g.crysene, benzo(a)pyrene, pyrene, benzo(k)fluoranthene etc.) in comparable conditions.
Thanks in advance!!!
Merja from Finland
By Einar Pontén - SeQuant AB on Thursday, January 23, 2003 - 12:31 pm:
There are several papers demonstrating this. Send me an e-mail and I will try to help you. I also think that we may have some perylene in our lab, as well as nitro- and aminoderivatives.
By Uwe Neue on Thursday, January 23, 2003 - 02:58 pm:
Retention times of PAH's change with the nature of the column. If your column is the type that has been designed for PAH separations, you should be able to get a test chromatogram from the column supplier. If this is not a PAH column, the retention times of some of these analytes might change with the nature of the column. See publications by Sander and Wise for reference.
By Uwe Neue on Thursday, January 23, 2003 - 06:06 pm:
Here are some references: LC Sander, SA Wise, J. Chromatogr. A 656, 335-351 (1993) and Anal Chem 67, 3284-3292 (1995)