Hallo

I will soon go to the wonderful world of biological samples (blood, serum, plasma etc.). I was wondering if you could give some refs or web pages which deal with sample prep. for HPLC. There are tons of papers with informations on this subject, and i would like to fish out the best ones with as much info as possible.

regards

Check at www.waters.com and look for methods using Oasis sample preparation. On-line or off-line plasma, with LC/MS or LC-UV.

Hello.

I'm in the wonderful world of biological samples.

My sugest is that you have looking for the solid phase extraction methodologies for cleaning this types of samples

Hallo,
since we are talking about biological samples, which are also new to me, perhaps someone could help me with the following:
I will be developing a method for drug in suction blister fluid, and i am not sure what shall I use as a matrix for blank and spiked samples

thanks for suggestions

Take a look at the Cohesive Technologies. Turboflow columns. These allow for the direct injection of biological material onto the HPLC system without the need for much sample preparation (just a bit of centrifugation and pH adjustment).

For suction blisters you could use serum.

in-line sample preparation can be completed with a dialysis unit. This unit can be used for blood / urine / emulsions / suspensions.
tremendous reduction in time and effort for sample prep ....
the sample is prepared from the sample changer to the injection valve....
A sample can be prepared, while an other sample is running ... see these links for details
http://metrohm.com/products/07/833/dialysis/833dia.html

http://metrohm.com/basics/encyc/ic/dialysis.htm

http://metrohm.com/applications/ic/misp_index.html
it is worth investigating