By Anonymous on Thursday, February 27, 2003 - 01:16 pm:
has anyone found a good alternative to an xterra C18
By Chris Pohl on Thursday, February 27, 2003 - 01:34 pm:
Specifically which chromatographic property or properties are you interested in finding an analog for in another product? PH stability?
By Anonymous on Friday, February 28, 2003 - 07:46 am:
Why should you take an alternative?
If I found a columns that matches my specs, I would only change if the columns isn't supplied anymore.
By Benjamin on Friday, February 28, 2003 - 11:50 am:
Dear Anonymous 1;
I have done some comparison separating biomolecules of about 5k daltons in the Xterra MS C18 and the Diazem Peptica C18-D. Both have performed very much the same, and the Peptica column is cheaper.
Good Luck;
Benjamin
By Frank on Sunday, March 2, 2003 - 11:20 pm:
Dear Benjamin,
the Peptica might be cheaper...but there are other points to consider.
The Xterra MS shows very little bleeding, this might be an advantage when doing MS-Detection. Another point is life-time of the column. I am using all kinds of Xterra and I have made thousands of injections even at low pH as 1.5 and they all are still doing their job.
Hope this helps...
Frank
By Anonymous on Tuesday, March 4, 2003 - 11:33 am:
As far as an alternative for XTerra goes, there really isn't one at the moment. The original questions still remains: Why are you looking for an alternative?
By Anonymous on Tuesday, March 4, 2003 - 12:31 pm:
It seems most of participant of this discussion including originator are Waters' men.
Once we've purchased a Pico-Tag column from Waters which voided badly after less than 100 injections.
By frank on Tuesday, March 4, 2003 - 11:46 pm:
To Anonymous from March 4, 2003 - 12:31 pm
No, I 'm not a Waters Rep.
But I do have my experience with Waters columns.
Concerning the Pico-Tag column from Waters, it seems man typically remembers only the bad things in life. What about other vendors??? I am using the Atlantis from Waters for some time now and...OK..i have had one column out of many that voided badly after 200 injections (it was always 0.1% TFA in water). But there was no real alternative to this method...
Life isn't always only black or white...
Frank
By Anonymous on Wednesday, March 5, 2003 - 06:32 am:
Hi Anonymous #1
if you search for a column that work at high pH (e.g. pH=11) you can try Zorbax Extend.
P.S. I'm not an Agilent' man. I have only tried Xterra and Zorbax Extend with good results for both columns.