By ebadi on Wednesday, April 2, 2003 - 10:57 am:
Does anyone have work with the Zorbax SB-phenyl column?
I am running a mobile phase 67% buffer (formate pH=3.6) 33% ACN.
The column is very sensitive to mobile phase composition. It takes about half a day to equilibrate and I lost my resolution only after 10 sample injection
By Russ on Thursday, April 3, 2003 - 05:45 am:
We have not had the problem you are mentioning. The equilibration times are similar to our C18 columns and we do not have a problem with slight mobile phase composition changes. We do not use a "true" buffer; our aqueous phase is 0.5 % HOAc in water. Don't know if your analyte is sensitive to slight changes in the buffer or not. Do you actually measure the buffer pH? If so, what is your allowable range?
By mike on Thursday, April 3, 2003 - 09:41 am:
This is an development work. I was toold that the column is sensitive to ACN and I should us meOH instead.
By Anonymous on Tuesday, April 8, 2003 - 07:53 am:
I would recommend calling the LC Column Tech Support group at Agilent since they are the maufacturer of Zorbax LC columns. I've called them a number of times and they were really helpful. They were able to help me with problems I had been struggling with for days. Give them a call at 800-227-9770, Option #4 then option #2. Good luck!!
By M_Gardner on Wednesday, April 16, 2003 - 02:11 pm:
I used this column for a quantitative assay with an LC/MS/MS method. It was the only one I found that would baseline resolve my analyte from a matrix interferent in a reasonable amount of time. Even though the samples were very dirty, I never had problems with lost resolution or long equilibration times, with sets of 30+ samples. I did use a guard column, of course.