By Anonymous on Wednesday, April 9, 2003 - 03:46 pm:
Hi,
What kind of problems would you expect to see when working with a drug in rat plasma, in comparaison to human plasma? I believe esterase in rat plasma could bring stability issues, but what else? How would you treat your plasma prior to extraction or for chromatography? Would you expect more matrix effect? Thanks for your comments.
By Anonymous on Wednesday, April 9, 2003 - 07:16 pm:
Obviously, rat plasma is not human plasma, but the general problems are the same. There is no difference in enzyme activity between human plasma and rat plasma.
By greg kovac on Thursday, April 10, 2003 - 11:28 am:
First you must break interaction protein-Analyte
usually by acidifing your sample.
THen,you must separate your analyte and eliminate the interference.May be solid phase extraction methods would be good.
Please,be specific with your compound of interest,and maybe many persons can you tell about how you could work this.
By HW Mueller on Thursday, April 10, 2003 - 11:54 pm:
2nd Anon is right regarding similarity in methods, but I am not sure about comparative enzyme composition. Though, if there are differences they are experimentally immaterial as you have to check the stability of your compound in the plasma anyway.
On the workup see "Precipitation vs. filtration for amino acid analysis....", April 4. I suspect that there are as many compounds that find denatured (acidified) proteins as "attractive" as compounds which show an affinity to natural proteins.