By Anonymous on Sunday, April 13, 2003 - 11:25 am:
Hello All,
I am looking for a HPLC separation of Vitamine B1 TPP and Vitamin B6 PLP in one HPLC run.
Anyone got any suggestions.
Thanx
By alex on Monday, April 14, 2003 - 12:25 am:
Most column vendors have an application library. "water soluble vitamins" is a standard application that is included in most cases.
By C.Sychov on Wednesday, April 23, 2003 - 11:48 am:
Stanndard system C18 phase, eluent water+Et3N+heptsulf+acid to ph3 -- acetonitrile.
I think itis the best. In other i agree with alex - just find the details "in press" :)
By Anonymous on Sunday, April 27, 2003 - 10:01 pm:
Try Waters phenyl 300*4.6mm column
with 50 mM hexane 1 sulphonic acid in 1% acetic acid: methanol: acetonitrile(80:15:5) you will get good separation B6 followed by B1.
or use any good c 18.
By Anonymous on Thursday, May 8, 2003 - 10:47 am:
I've used the Alltech Prevail C18, which is water-wetable. Using 97% phosphate buffer at pH 3.0 and 3% acetonitrile and it has worked quite well. Alltech has an application number 9388 which should help. Try www.alltechweb.com
By Anonymous on Thursday, July 10, 2003 - 06:50 am:
Alltech has a Prevail C18 column designed to analyze water soluble vitamins and amino acids in one run.
By Anonymous on Friday, May 14, 2004 - 11:07 am:
Hi All,
I want an HPLC method for estimation of water soluble vitamins in meat and milk.