By frank poetsch on Tuesday, May 6, 2003 - 11:20 pm:
Dear board readers,
I am desperately searching for a method to determine the free fatty acids in saw palmetto by HPLC with ELSD detection.
Does someone know where to find literature about this analytical problem?
To avoid the logical answer: I already have done it by GC (FAMEs), HPLC after derivatization won't do very well.
I need this for phytoequivalence studies of different saw palmetto extracts.
Thanks alot in advance.
Frank
By Anonymous on Wednesday, May 7, 2003 - 06:13 am:
Hi Frank,
have you already tried www.nutraceuticalinstitute.com?
By Anonymous on Wednesday, May 7, 2003 - 06:31 am:
Sorry Frank,
I have verified that Institute for Nutraceutical Advancement is not present as www.nutraceuticalinstitute.com
I have not found the actual address
By Frank on Sunday, May 11, 2003 - 10:54 pm:
Yes, I have already checked the INA website...
Unfortunately, you can only find a method done by GC...No HPLC method can be found.
Any other ideas???
By the way...the current adress of the INA is www.nsfina.org
By ves on Friday, August 1, 2003 - 06:08 am:
http://www.cardinal.com/pts/content/aboutus/whoweare/broch/HPLC.pdf
Experimental
• 800 mg of saw palmetto extract is first dissolved and diluted to 250 mL with 2-
propanol.
• 1.0 mL of this solution is transferred to a 10 mL volumetric flask. The free
fatty acids are neutralized by adding 1 mL of 0.1N methanolic KOH.
• 1.0 mL of 0.03 N dicyclohexano-18-crown-6 solution is added to sequester the
K+, preventing precipitation of KOH.
• 1 mL of 0.3 M Dibromoacetophenone is added to the solution - at least a 20
fold excess.
• This solution is diluted to volume with acetonitrile.
• An aliquot is transferred to a reaction vial and heated at 80o C for 1 hour.
• Higher temperatures can lead to saponification of triglycerides.
By ves on Friday, August 1, 2003 - 06:22 am:
Alltech has a technical library at this address. Several tech papers on hplc/elsd with one specifically for underivitized fatty acids. Not too much on the extraction, though.
http://www.alltechweb.com/US/Tech_Library.asp?type=4
By Frank on Monday, August 25, 2003 - 02:12 am:
Back from holidays I 'd like to thank you for you support...
Frank