I WAS WONDERING IF ANYONE HAS EXPERIANCE WITH THE DETECTION OF POLYAMINES ( SPERMINE, SPERMIDINE, OR PUTRACINE USING HPLC. WHAT DETECTOR AND COLUMN MIGHT BE REQUIRED.

i FORGOT TO MENTION HOW SENSITIVE IS THE DETECTION?

Dionex sells a column which works for these analytes (IonPac CS17). Analytes can be detected by conductivity or amperometry. Choice of detector is somewhat dependent on sensitivity requirements. What sort of detection limit do you require?

Thanks Cris! We need the most sensitive technique that we can get. I usually like to us an injeection volume of no more than 20 ul and were working with a small number of cell like in the order of 10,000. Al

It's a bit more complex but good results can be obtained with dansyl chloride derivatization and reverse phase HPLC on C18 column. This method is well established for histamine analysis and I have verified (many years ago) that is suitable for polyamines (putrescine, cadaverine, spermidine, spermine) analysis.
Dansyl moiety allow you to use UV detection or fluorometric detection if you need high sensitivity.

Al,

Using the suppressed conductivity method with a 2 mm ID column, the detection limit for spermine is around 1 pM. Detection limits are similar for other analytes. Electrochemical detection gives slightly better sensitivity but is more sensitive to eluent composition and so because this is a gradient method actual detection limits are somewhat better using conductivity detection all things being considered. If you need better sensitivity than this, a practical option would be o-phthaldehyde post-column reaction with fluorescence detection.

I certainly would try a ZIC™-HILIC column.
Contact The Nest Group for more information.

Dansyl derivatives can be also well resolved on silica, eluent hexane-ethylacetate.

RATHER USING DANSYL DERIVATES WHICH REQUIRE SPECIAL GLASSWARE, COVERED WITH SILICA, AND ARE RELATIVELY UNSTABLE I SUGGEST DABSYL-CHLORIDE OR FNDB AS DERIVATIZING AGENT. THE LATTER ONE IS FAR MORE STABLE THAN ANY OTHER DERIVATIZING REAGENT.
IF YOU WANT THE BEST SENSITIVITY THAT YOU CAN GET THEN DABSYL-CHLORIDE IS YOUR CHOICE.
I KNOW IT SOUNDS STRANGE BUT THE D.L USING DABS-Cl AS DERIVATIZING AGENT IS LOWER THAN THE D.L IN THE CASE OF DANSYL DERIVATIVES.
I THINK THAT SUPELCO IN ITS CATALOQUE SUGGESTS THE USE OF DABSYL-DERIVATIVES TO ANALYSE BIOGENIC AMINES AFTER DERIVATIZATION WITH THA REAGENT

Thanks for all your help. Will keep in touch. Your a great bunch. Al

I just want to know how to prepare the pancreas tissues sample for polyamine HPLC measurement . And how to store the samples , storage conditions like temperature and so on . Any help will be appreciated !

i wonder what the best mobile phase composition would be for separation of biogenic amines on an hplc c18 column. using phosphate buffer with sds and 2 propanol doesnt seem to elute them under an hour