i am developing a method of analysis for a odt tablet which contains
1) pepperming flavour
2) aniseed flavour
3) tutti fruity flavour
4) aspartam

i am using following method

mobile phase A: buffer having 5.18gm of sodium acetate ,1.0452gm of tetrabutyl ammonium hydrogen sulphate in 1000ml water.

mobile phase B : 200BUFFER ABOVE +800METHANOL

dILUENT: 1:1 MIXTURE OF A:B

I AM USING DISCOVERY HS C18
250X 4.6 5um column
flow rate is 1.5ml/min

gradient programme is as follows

time A B

0.01 65 35

25.0 30 70

35.0 15 85

36.0 65 35

45.0 65 35

my sample tablet active is having 7to 8 impurities including 3 degradants, whats happening is that i am getting the flavours peaks at the same time of my impurities peaks and also so many peaks due to comibined placebo of all this with mannitol.

my blank is absolutely clear no peaks are coming

i would like to know sir JOHN what strategy i can approach now , wheather i should go for some new alternate method , or can we deactivate the flavour anyhow so that it can not give any peaks

shirish
shi_ri@indiatimes.com