By Lucy on Thursday, June 12, 2003 - 02:04 am:
Yes, I agree, this is a great discussion group.
I have been set (or dropped!) the task of setting up an HPLC in our research lab (where I work) from an old system used previously.
I have quite a bit of HPLC experience, but have never set one up by myself before.
Does anyone have any hints/tips about practical considerations in setting one up?
Any help would be most welcome.
By Anonymous on Thursday, June 12, 2003 - 05:41 am:
what manufacturer ?
they all have particular things that you will need to address.
method of degas ?
do you need air for pneumatic valves ?
give me the model #s....
By Lucy on Thursday, June 12, 2003 - 06:59 am:
The equipment I have is:
>Perkin Elmer 100 series pump
>Anachem column heater with Rheodne injector
>Perkin Elmer LC90 Sepc detector
I haven't got that much of an idea as to how to start, we haven't got helium into our lab, so I was considering vacuum filtration.
Presumably, as my equipment seems quite "dated" I am not sure whether the valves are pneumatic.
I am hoping to try to interface it through a PE 900 series interface box, to either Turbo-chrom/Totalchrom for data handling.
By Lucy on Sunday, June 15, 2003 - 10:58 am:
I would be grateful for any more assistance/expertise!
By Anonymous on Thursday, June 19, 2003 - 08:44 am:
It really would not be too hard to do. The solvent outlet from the pump would go into port #2 on the rheodyne injector. The outlet from the injector would be port #3(to column), and this of course should stay in the col. heater box. Outlet from column goes to the detector inlet and detector outlet to waste. The electrical hookups would depend on the injector. Is there a contact closure output from the injector? If so, this would be hooked to the ext. inj. port on the series 200 pump(you will need a special connector). The inject output from the pump will then go to the 900 box for the start in(you will need a special connector) at the beginning of a run. The detector 1 volt output(or sometimes labeled computer output) will go into the channel A + and - on the 900 box(top connector). Connect the box, either serial or IEEE, to the TCWS computer, configure the software to use the box, and you should be set.