By Anonymous on Monday, June 23, 2003 - 06:17 am:
Hi,
I'm absolutely rubbish at maths, so could anone help please?
I have the average peak height (in microamps) and an assayed percentage of two components, and I also have the average peak height of the two components in a sample. I have the masses of both standard and sample.
How can I calculate the percentage of both components in the sample from the information I have from the standard and sample?
By Michael on Tuesday, June 24, 2003 - 07:35 am:
A few questions first. What type detector are you using? Does this detector have equal sensitivity for both standard and sample (the analyte)?
So, if equal amounts of standard and analyte were injected, would the detector give the same peak height for both?
Are you trying to calculate percent composition of the analyte in your whole sample (ie. % vs solvent, matrix, and standard) or just a ratio of analyte to standard?
By Anonymous on Wednesday, June 25, 2003 - 01:18 am:
>A few questions first. What type detector are >you using? Does this detector have equal >sensitivity for both standard and sample (the >analyte)?
A diode array detector. The standard is the same matrix as the sample
>So, if equal amounts of standard and analyte >were injected, would the detector give the same >peak height for both?
Yes
>Are you trying to calculate percent composition >of the analyte in your whole sample (ie. % vs >solvent, matrix, and standard) or just a ratio >of analyte to standard?
Trying to calculate both