Purification of an glycoprotein, which is present in proteinfree medium

Chromatography Forum: LC Archives: Purification of an glycoprotein, which is present in proteinfree medium
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Thursday, June 26, 2003 - 12:35 am:

Hi,

can anyone help me? I'm trying to purify a glycoprotein from the supernatant of cells. The protein is present in protein-free medium. It binds to strong anion exchange column. I have not tried many columns. Can you help me in finding the right columns to use? Which one should I used first? I have an FPLC from Biorad.


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