By John on Thursday, July 3, 2003 - 01:41 am:
Dear Forum Members,
I have an drug molecule which is deuteriated (induced with deuterium). During my synthesis process the purity of the Deuteriated form is just 90%, other 10% is undeuteriated form of the same molecule. I want to purify and eliminate the 10% undeuteriated form from my product. Are there any HPLC columns / applications available to perform the task or any conventional methods to purify the same. Awaiting for your valuable responses.
By Uwe Neue on Thursday, July 3, 2003 - 03:09 pm:
The selectivity difference between a deuterated and a hydrogenated compound are very small, and the chances are that the separation by RP will be only very slight - not even two peaks.
By HW Mueller on Thursday, July 3, 2003 - 11:29 pm:
There are unbelievable separations of D-compounds in GC, probably nobody would suspect these would separate in HPLC.