By Anonymous on Tuesday, July 29, 2003 - 01:53 pm:
Does anyone know what this is? As I have to develop a method so
By Anonymous on Tuesday, July 29, 2003 - 06:38 pm:
Most who frequent this site would know what a derivitive is and possibly how to make one. I would suggest to you to open up a basic chemistry textbook and try reading it.
By Anonymous on Wednesday, July 30, 2003 - 12:21 am:
I have no chemistry books round, I just wanted a basic understanding
By Anonymous on Wednesday, July 30, 2003 - 02:24 am:
If you can't separate or detect something, you can convert it to something else by chemical reaction. This is called derivatisation.
By Anonymous on Wednesday, July 30, 2003 - 04:20 am:
Do you know where I could go about looking for how to do this? I have a normal phase column and need to separate phenoxy acetic acid from phenol
By Anonymous on Wednesday, July 30, 2003 - 04:46 am:
google is a good place to start
By Chris Pohl on Wednesday, July 30, 2003 - 08:22 am:
What makes you think that you need to derivatise these compounds? They are both easily detected using a UV detector. If you're interested in boosting the sensitivity for phenol, you can accomplish this by adding base through a mixing tee to convert the phenol to its ionized form which has a significantly higher extinction coefficient.
By Anonymous on Wednesday, July 30, 2003 - 10:22 am:
Oh thank you, does anoyne know where I can find a method for this separation? Als, would running this on GC DB5 damage it?
By Anonymous on Monday, August 18, 2003 - 08:26 am:
DB5 is not the column of choice for polar compounds in GC. I would stay within LC. UV detection gives high sensitivity.I don't see much problems.Phenol is a quick eluting compound in RP separation,the acid should retain longer.I would try C18 with an acidic eluent.Alternatively perhaps Hypercarb.
PETER
By Anonymous on Thursday, June 10, 2004 - 12:08 am:
If anybody could assist I would appreciate it. I need to separate cis-retinol and trans-retanol on a C18 column. All the info I have explored thus far refers to using a normal phase column to separate the isomers of vitamin A. Large pore C18 silicas do not exclude long rigid molecules such as vitamin A, therefor they(C18 phases 300A)should provide better selectivity. My question relates to what column to use. I am curently using a Agilent 1100 HPLC