By Anonymous on Wednesday, August 6, 2003 - 10:48 pm:
what is the necessity to dissolve the sample in Mobile phase (as in different pharmacopie)?
By Anonymous on Wednesday, August 6, 2003 - 11:45 pm:
to prevents mixing effects between mobile and injection solvent (peak broadening, solvent peaks..)
By M_Gardner on Thursday, August 7, 2003 - 06:32 am:
A solvent with stronger eluting strength than your mobile phase will act like a strong mobile phase briefly when it hits the head of your column, causing the problems mentioned by anon # 2. Your sample doesn't have to be dissolved in your starting mobile phase, but if not, it should be dissolved in a *weaker* solvent than your starting mobile phase.