By Anonymous on Friday, August 8, 2003 - 12:47 am:
Does anyone know where I can find a method/has a method for the analysis of phenoxyacetic acid by HPLC and quantification? E.G would this be normal phase or perhap Dionex?
By Anonymous on Friday, August 8, 2003 - 05:59 am:
Dear Anon,
If you are after trace levels check with the US EPA, they have a good method or two. If you are analyzing a formulation of the neat material I would use reverse-phase LC with some TFA in the mobile phase to suppress the ionization and analyze the molecular species. I don't have a reference, just my experience in method development.
Regards,
Mark
By M_Gardner on Friday, August 8, 2003 - 06:29 am:
2,4-dichlorophenoxyacetic acid chromatographs just fine on an endcapped C18 with 0.2% acetic acid in the mobile phase.
By Anonymous on Monday, August 11, 2003 - 03:39 am:
I have separated 2,4-D and MCPA (and other acidic herbicides) with C18 endcapped column (Purospher Merck) using a gradient acetonitrile/water both with 1 mM formic acid.
good luck
By Anonymous on Tuesday, August 19, 2003 - 02:45 am:
I think this is the second call for papers (the first one:29./30 july).Isn't it help enough to say RP 18 under acidic conditions? To be more precise I would take intuitively 30-40% ACN/water pH 3-4.The rest is y o u r job.
PETER
By Mahaveer on Saturday, July 10, 2004 - 09:10 pm:
How to separate phenoxy acetic acid herbicides by HPLC in an isocratic mode ?