By Anonymous on Thursday, August 28, 2003 - 09:13 am:
I am developing an SEC aggregation assay for a protein therapeutic. My question revolves around the choice of mobile phase. Is it common practice to use the formulation buffer as the mobile phase or can a standard phosphate buffer be used? I don't want to introduce artifacts by either introducing or disrupting aggregation during the assay but I don't want to constantly requalify an assay upon formulation changes.
By Anonymous on Thursday, August 28, 2003 - 05:24 pm:
I would use the formulation buffer, if it is compatible with SEC. If you use another buffer, aggregation could be caused or broken by the mobile phase buffer, and you know nothing.
By HW Mueller on Tuesday, September 2, 2003 - 04:17 am:
Even if you use your formulation buffer, doesn´t the stat. phase cause a disruption of your aggregates?
By Uwe Neue on Tuesday, September 2, 2003 - 03:19 pm:
HW: in SEC?
By HW Mueller on Wednesday, September 3, 2003 - 02:34 am:
I have not been fortunate enough to have had a perfect SEC column yet.
By Uwe Neue on Friday, September 5, 2003 - 01:43 pm:
There will be a publication in the Application Notebook Supplement of LC/GC in September on this subject. It shows the SEC-MS analysis of aggregates in protein mixtures (as the title says). If you do it right and use a decent column, it works.
By mgb on Friday, September 5, 2003 - 02:37 pm:
Is dynamic light scattering a possibility for your assay. This will be non-invasive.