By Fabiano on Tuesday, September 2, 2003 - 09:14 am:
Someone use polimeric reverse-phase columns at basic pH instead of the boring SDS ion-pair with post column derivatization for amines?
My analytes are amines up to 8 carbons, all aliphatic.
Fabiano
By Uwe Neue on Tuesday, September 2, 2003 - 03:38 pm:
We have used XTerra columns at alkaline pH (usually 10) for many basic compounds, mostly amines. The retention is between 10- and 30-fold higher compared to acidic pH.
By Anonymous on Wednesday, September 3, 2003 - 01:24 am:
You can also use pre column dansylation (see Price et al. (1992).J.Chromatogr. 598:51-57.
Chromatography itself is then easily performed on a classical C18 phase using a water/CH3CN gradient.
By Fabiano on Wednesday, September 3, 2003 - 04:03 am:
To Uwe:
How long the column withstood to basic pH? I mean, it showed any degradation?
By Uwe Neue on Wednesday, September 3, 2003 - 10:27 am:
45 days at pH 11.5 at 30 degrees at 1 mL/min around the clock.