Can I detect this by UV?

Chromatography Forum: LC Archives: Can I detect this by UV?
Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Tuesday, May 18, 1999 - 10:39 am:

I am interested in detecting Ethyl Bicyclic Phosphate cas# 1005-93-2. The only double bond present is between Oxygen and Phosphorus. Is it detectable by UV?


Top of pagePrevious messageNext messageBottom of pageLink to this message  By Anonymous on Thursday, May 20, 1999 - 10:13 am:

In theroy, there may be electronic transitions between the non-bonded electrons on oxygen to a pi-star orbital, similar to what occurs in C=O, S=O and N=O systems. The wavelength may be anywhere from 215 - 300 nm, although the absorptivity is likely to be low for this functional group. I successfully detected a compound with structure R-P=O(OH)-R at 215 nm in phosphate buffer and acetonitrile.

In practice, a good way to find out would be to perform a wavelength scan on this material in your HPLC solvent in a UV/VIS scpectrometer. This would also give you an idea of the wavelength to use for detection.


Posting is currently disabled in this topic. Contact your discussion moderator for more information.